Gonococcal PorB is a major outer membrane porin important for nutrient acquisition, antimicrobial resistance, and immune modulation. PorB is the most abundant protein in outer membrane vesicles (OMVs) of Neisseria gonorrhoeae and other Neisseria species. OMVs from N. meningitidis are a component of a Neisseria meningitidis vaccine known to induce immune responses against N. gonorrhoeae, which has prompted investigation of N. gonorrhoeae OMVs as a potential component of a gonococcal vaccine. Unfortunately, gonococcal PorB suppresses dendritic cell-mediated T-cell proliferation, which may limit the utility of an OMV-containing vaccine. Therefore, we aimed to minimize the suppressive effects of PorB in OMVs by reducing or eliminating PorB in the gonococci. However, unlike N. meningitidis, which remains viable when its two porins, PorA and PorB, are deleted, N. gonorrhoeae PorB is essential for growth and cannot be deleted. To overcome this limitation, we generated a porB-CRISPRi strain using an inducible CRISPRi knock-down system developed by Geslewitz et al. (2024) to decrease PorB protein levels in whole cells and in OMVs derived from this strain. PorB levels were markedly reduced in cells grown in liquid media with IPTG relative to the same cells grown without IPTG, as determined by Western blotting with a PorB-specific mAb. The growth of these cells, as measured by Area Under the Curve (OD600), was reduced by ~33% in GCB but not reduced when grown in Graver-Wade medium. Scanning Electron Microscopy (SEM) images of induced cultures in GCB showed that the OMVs produced by this strain are heterogenous in size and attached together in irregular patterns. Additionally, porB-CRISPRi cells with low levels of PorB are clumped, display irregular diplococcal formation, and have a much smoother surface relative to normal or uninduced cells. Current directions include examining the effects of PilQ (the pilus secretin with some pore activity) on the viability of the induced porB-CRISPRi strain and quantifying the impact of reducing levels of PorB on antibiotic resistance and dendritic cell-mediated proliferation of T cells.